eng Rovedar Research in Biotechnology and Environmental Science 2980-7743 2024-06-25 3 2 23 28 10.58803/rbes.v3i2.38 40 Yamuna Devi 0 https://orcid.org/0000-0001-5206-0580 Appasamy Surendran 1 https://orcid.org/0009-0008-9931-681X Antony Joseph Thatheyus 2 https://orcid.org/0000-0001-5206-0580 1 Department of Zoology, The American College, Madurai, Tamil Nadu, India 2 Department of Biochemistry, The American College, Madurai, Tamil Nadu, India 3 Department of Microbiology, The American College, Madurai, Tamil Nadu, India Introduction: Phytoremediation is a cutting-edge and eco-friendly technique that utilizes plants to eliminate pollutants, including copper, which can pose risks to plants, animals, and humans. In the present study, the hyperaccumulator capabilities of the tomato plant (Lycopersicon esculentum) in the removal of copper, zinc, iron, and manganese from the soil was explored. Materials and Methods: The tomato plants were cultivated for 60 days in pots containing varying concentrations of copper, ranging from 250 to 1250 ppm. At specific time intervals of 15, 30, 45, and 60 days, plants of each concentration were harvested. Then the soils were analyzed using atomic absorption spectroscopy to determine the levels of copper, zinc, manganese, and iron. Results: The results indicated that zinc removal exhibited a higher rate compared to other metals, with a removal rate of up to 95.79%, while copper removal reached 87.7%. Furthermore, analysis after 60 days of treatment revealed that the aerial parts of the plants accumulated more metals than that of the roots. Additionally, the chlorophyll content in the leaves decreased at both low and high copper concentrations, compared to 500 ppm CuSo4. Conclusion: The tomato plant, L. esculentum indicated promising hyperaccumulator potential in the removal of copper, zinc, iron, and manganese. The current study emphasized the effectiveness of phytoremediation as a sustainable approach to abating copper pollution. https://rbes.rovedar.com/index.php/RBES/article/view/38 Hyperaccumulator Lycopersicon esculentum Phytoremediation