_1705819875 20240121065115000 Rovedar Daryoushbabazadeh@gmail.com Rovedar Research in Biotechnology and Environmental Science Res. Biotechnol. Environ. Sci. 2980-7743 12 25 2023 2 4 Sabitha Appasamy Surendran https://orcid.org/0009-0008-9931-681X Antony Joseph Thatheyus https://orcid.org/0000-0001-5206-0580 Introduction: Pyrethroid pesticides are applied in agricultural fields to protect crops from pests and their residues, which can adversely affect soil and water quality, causing damage to non-target organisms. This research aimed to explore the potential role of the bacterial strain, Pseudomonas stutzeri in breaking down the pesticide,  Bifenthrin. Materials and Methods: The study focused on evaluating the efficiency of the bacterium, Pseudomonas stutzeri (MTCC2300) in degrading the pyrethroid, Bifenthrin. Various concentrations of Bifenthrin (2500, 5000, 7500, and 10000 ppm) were subjected to treatment with the bacterial strain in minimal broth for 16 days. Results: When the efficiency of Pseudomonas stutzeri on the degradation of 2500, 5000, 7500, and 10000 ppm of Bifenthrin was tested for a period of 16 days, a decrease in pH, and an increase in CO2, NH3, and biomass were observed. The pH was reduced to 7.6 while CO2 increased to 4 mg/ml, NH3 up to 0.8mM, and Biomass up to 0.6 g dry wt./ml. In a two-way ANOVA, Bifenthrin concentration resulted in a statistically significant variation in parameters like, pH, CO2, and NH3 of the culture medium. 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