_1713417454 20240418094734000 Rovedar Daryoushbabazadeh@gmail.com Rovedar Research in Biotechnology and Environmental Science Res. Biotechnol. Environ. Sci. 2980-7743 03 28 2024 3 1 Dickson Onyenmulo Uwaya https://orcid.org/0000-0001-6217-4071 Offiong Nnom Effiong https://orcid.org/0009-0009-4862-2400 Introduction: Polyherbal formulations maximize therapeutic effects and reduce toxicity by combining effective herbs in specific ratios. The present study aimed to quantify some phytochemical constituents, and some non-enzymatic antioxidants and to estimate the analgesic, expectorant, and antitussive properties of polyherbal-formulated tea (Curcuma longa, Citrus limon, Zingiber officinale, Allium sativum, and Moringa oleifera). Materials and Methods: Some phytochemical constituents and some non-enzymatic antioxidants of the polyherbal tea were evaluated using colorimetric methods. The antitussive efficacy was assessed by examining the cough induced by citric acid in 20 healthy guinea pigs and ammonium in 20 mice. The expectorant activity was evaluated using phenol dye secretion in mice. The analgesic properties were analyzed using pain caused by a hot plate and writhing test caused by acetic acid. Four groups were formed by randomly dividing 20 healthy adult experimental animals (mice and guinea pig), with 5 of both sexes’ animals in each group. Group 1 was given distilled water (10 ml/kg), group 2 was given 5 mg/kg of the polyherbal-formulated tea, group 3 was given 10 mg/kg of the polyherbal-formulated tea, and group 4 was given standard drugs depending on the model of animals used. The tea and standard drugs were administered orally. Results: The result showed that the polyherbal-formulated tea contains phenolic compounds (53.57±1.96 mg/g), alkaloids (40.93±5.96 mg/g), flavonoids (99.44±1.96mg/g), Vitamin C (862±18.76mg/g), carotenoid (5200±6.93 mg/g) and Lycopene (19.50±1.35mg/g). The polyherbal-formulated tea decreased the number of cough bouts and raised the percentage of cough suppression caused by citric acid when compared to the control group. Tea decreased the number of cough bouts caused by ammonium in mice compared to the control group and it raised phenol dye secretion in the expectorant experiment. In hot plate-induced pain, tea increased the latency of the pain threshold in mice and reduced the number of writhing the percentage of pain inhibition increased compared to the control group in acetic acid-induced pain. Conclusion: The polyherbal-formulated tea contains phenolic compounds, alkaloids, flavonoids, Vitamin C, carotenoid, and lycopene and has antitussive, expectorant, and analgesic activity. 03 30 2024 9 17 https://creativecommons.org/licenses/by/4.0 10.58803/rbes.v3i1.29 https://rbes.rovedar.com/index.php/RBES/article/view/29 https://rbes.rovedar.com/index.php/RBES/article/download/29/70 https://rbes.rovedar.com/index.php/RBES/article/download/29/70 10.5694/j.1326-5377.2006.tb00548.x 10.1007/978-3-642-98093-0 10.31080/ASAG.2019.03.0683 10.1080/10408398.2015.1077195 10.3767/000651904X484432 10.1016/j.jep.2014.11.041 10.1186/s13002-015-0052-0 10.1177/1934578X1100601012 10.1002/ptr.2023 10.3390/plants10020318 10.1109/ACCESS.2018.2793851 10.7897/2230-8407.0507106 10.1016/j.jnutbio.2017.10.010 10.1186/s12906-016-1443-z Shazia T, Swati K, and Kirti J. Spectrophotometric quantification of total phenolic, flavonoid, and alkaloid contents of abrus Precatorius L. seeds. Asian J Pharm Clin Res. 2016; 9(2): 371-374. Available at: https://journals.innovareacademics.in/index.php/ajpcr/article/view/11018 Mallick CP, and Singh MB. Plant enzymology and histo enzymology. New Delhi: Kalyani publishers; 1980. 10.1016/0304-4165(79)90289-7 Palghat RP, and Matheswaran J. Comparison of enzymic and non-enzymic antioxidant status in leaves of five selected plants from apiaceae family. Ijppr Human, 2016; 6(3): 824-828. Available at: https://ijppr.humanjournals.com/wp-content/uploads/2016/07/1. Matheswaran-Jagathambal-and-Palghat-Raghunathan-Padma.pdf 10.1016/j.cbi.2020.109211 10.4314/jasem.v27i8.35 10.52406/ptnm.v2i4-6.47 Samatha T, Shyamsundarachary R, Srinivas P, and Swamy NR. Quantification of total phenolic and total flavonoid contents in extracts of Oroxylum indicum L. Kurz. Asian J Pharm Clin Res. 2012; 5(4): 177-179. 10.3109/13880209.2013.872672 Seth SD. and Sharma B. Medicinal plants in India. Indian J Med Res. 2004; 120(1): 9-11. Available at: https://pubmed.ncbi.nlm.nih.gov/ 15299226/ 10.5772/intechopen.69847 Sultana S, Khan A, Safhi MM. and Alhazmi HA. Cough suppressant herbal drugs: A review. Int J Pharm Sci Invent. 2016; 5(5): 15-28. Available at: https://www.ijpsi.org/Papers/Vol5(5)/D0505015028.pdf 10.1016/j.biopha.2018.10.058 10.4209/aaqr.2008.04.0012 10.1152/jappl.2001.91.6.2642 10.1152/ajplung.00353.2001 10.1038/sj.bjp.0706494 10.4103/0250-474X.45419 10.1056/NEJMoa043891 10.1016/j.jep.2011.10.036 10.9734/JSRR/2015/14245 10.1021/jm020969t 10.2174/1381612043453036 10.1002/jsfa.8628 10.1021/acs.jafc.7b04572 10.1038/sj.bjp.0705941 10.4062/biomolther.2010.18.1.092