_1720416918 20240708090518000 Rovedar Daryoushbabazadeh@gmail.com Rovedar Research in Biotechnology and Environmental Science Res. Biotechnol. Environ. Sci. 2980-7743 06 25 2024 3 2 Yamuna Devi https://orcid.org/0000-0001-5206-0580 Appasamy Surendran https://orcid.org/0009-0008-9931-681X Antony Joseph Thatheyus https://orcid.org/0000-0001-5206-0580 Introduction: Phytoremediation is a cutting-edge and eco-friendly technique that utilizes plants to eliminate pollutants, including copper, which can pose risks to plants, animals, and humans. In the present study, the hyperaccumulator capabilities of the tomato plant (Lycopersicon esculentum) in the removal of copper, zinc, iron, and manganese from the soil was explored. Materials and Methods: The tomato plants were cultivated for 60 days in pots containing varying concentrations of copper, ranging from 250 to 1250 ppm. At specific time intervals of 15, 30, 45, and 60 days, plants of each concentration were harvested. Then the soils were analyzed using atomic absorption spectroscopy to determine the levels of copper, zinc, manganese, and iron. Results: The results indicated that zinc removal exhibited a higher rate compared to other metals, with a removal rate of up to 95.79%, while copper removal reached 87.7%. Furthermore, analysis after 60 days of treatment revealed that the aerial parts of the plants accumulated more metals than that of the roots. Additionally, the chlorophyll content in the leaves decreased at both low and high copper concentrations, compared to 500 ppm CuSo4. Conclusion: The tomato plant, L. esculentum indicated promising hyperaccumulator potential in the removal of copper, zinc, iron, and manganese. The current study emphasized the effectiveness of phytoremediation as a sustainable approach to abating copper pollution. 06 25 2024 23 28 https://creativecommons.org/licenses/by/4.0 10.58803/rbes.v3i2.38 https://rbes.rovedar.com/index.php/RBES/article/view/38 https://rbes.rovedar.com/index.php/RBES/article/download/38/79 https://rbes.rovedar.com/index.php/RBES/article/download/38/79 10.59324/ejtas.2024.2(1).48 10.1016/j.chemosphere.2021.132369 10.1016/j.envadv.2022.100204 10.1007/s12517-021-08543-9 10.1016/j.eti.2017.08.002 10.1007/978-3-031-48817-7_1 10.1016/B978-0-12-821656-9.00004-3 10.1080/02757540.2023.2284158 10.1016/j.indic.2021.100155 10.1016/j.eti.2021.102114 10.1007/s10098-021-02029-8 10.1016/j.biortech.2021.124835 10.1016/j.asej.2020.05.009 10.1016/j.envres.2021.110780 10.1016/j.jhazmat.2020.123658 10.14302/issn.2641-4538.jphi-20-3475 10.11648/j.ijec.20190302.14 10.1002/9781119853589.ch2 10.1016/j.eti.2020.100672 10.1016/j.ecoleng.2018.05.039 10.1016/j.chemosphere.2016.12.116 Shabbir Z, Sardar A, Shabbir A, Abbas G, Shamshad S, Khalid S, et al. Copper uptake, essentiality, toxicity, detoxification and risk assessment in soil-plant environment. Chemosphere. 2020; 259: 127436. DOI: 10.1016/j.chemosphere.2020.127436 10.1016/j.chemosphere.2020.127810 10.1016/j.chemosphere.2020.127436 10.1016/j.envc.2021.100197 10.1111/nph.19070 10.1111/ppl.13285 10.1016/j.chemosphere.2019.05.098 10.1016/B978-0-12-819487-4.00006-9 10.1016/j.envpol.2022.119035 10.1016/j.chemosphere.2021.132979 10.1016/B978-0-12-385538-1.00004-4 10.1021/acs.jafc.9b04117 10.1186/s40538-014-0026-9 10.1007/s11157-013-9313-3