Screening of Biosurfactant-producing Bacteria from Symbiotic Microbes with Gastropods in the Persian Gulf

Main Article Content

Zeynab Bayat
Nazanin Akbari
Mehdi Hassanshahian
Simone Cappello
Ali Salehinasab

Abstract

Introduction: Biosurfactants or surface-active compounds with amphiphilic molecular structures, including a hydrophilic and a hydrophobic domain, are produced by microorganisms. These compounds increase the biodegradation of hydrocarbons in the environment due to their ability to emulsify hydrocarbon-water mixtures. This study was conducted to isolate and characterize biosurfactant-producing bacteria from the samples of Gastropods. 


Materials and Methods: The gastropod samples were collected from oil-contaminated sites in the Persian Gulf, Middle East. Biosurfactant-producing strains were isolated from these samples. The biosurfactant production ability was analyzed using Drop Collapse TEST, oil spreading test, emulsification activity test, and BATH test.


Results: In total, 11 biosurfactant-producing strains were isolated. Two isolates with higher growth rates and biosurfactant production ability were selected for further studies. The best isolates were identified as Halomonas sp. isolate BHA16 and Vibrio alginolyticus isolate BHA 17 based on molecular analysis. Gas chromatography analysis of remaining crude oil confirmed that these strains could degrade to 51.44 % and 67.58% of crude oil, respectively.


Conclusion: The results of this study indicated the surfactant activity of the bacterial strains isolated from Gastro pods had a good potential for the biodegradation of crude oil and could be used for the cleanup of oil-contaminated marine environments.

Article Details

How to Cite
Bayat, Z., Akbari, N., Hassanshahian, M., Cappello, S., & Salehinasab, A. (2022). Screening of Biosurfactant-producing Bacteria from Symbiotic Microbes with Gastropods in the Persian Gulf . Research in Biotechnology and Environmental Science, 1(1), 1–5. https://doi.org/10.58803/rbes.v1i1.1
Section
Original Article

References

Hussain T and Gondal MA. Monitoring and assessment of toxic metals in Gulf War oil spill contaminated soil using laser-induced breakdown spectroscopy. Environ Monit Assess. 2008; 136)1-3): 391-399. DOI: https://doi.org/10.1007/s10661-007-9694-2

Abdul Aziz AM, Adebayo AR, and Enamul Hossain M. A sustainable approach to controlling oil spills. J Environ Manage. 2012; 113: 213-227. DOI: https://doi.org/10.1016/j.jenvman.2012.07.034

Ahmad R, Sanghamitra P, and Mazumder D. Treatability of oily wastewater by anaerobic treatment system–A mini-review. J Indian

Chem Soc. 2020; 97(12b): 2859-2863. Available at: http://indianchemicalsociety.com/portal/uploads/journal/B-Dec-22.pdf

Kingston PF. Long-term environmental impact of oil spills. Spill

Scie Tech Bullet. 2002; 7(1-2): 53-61. DOI: https://doi.org/10.1016/S1353-2561(02)00051-8

Onwurah INE, Ogugua VN, Onyike NB, Ochonogor AE, and Otitoju OF. Crude oil spills in the environment, effects and some innovative cleanup biotechnologies. Int J Environ Res. 2007; 1(4): 307-320. Available at: https://www.sid.ir/paper/300644/en#downloadbottom

Bayat, Z, Hassanshahian M, and Cappello S. Immobilization of microbes for bioremediation of crude oil polluted environments:

A mini-review. Open Microbiol J. 2015; 9: 48-54. DOI: https://doi.org/10.2174%2F1874285801509010048

Almansoory AF, Abu Hasan H, Sheikh Abdullah SR, Idris M, Anuar N, and Al-Adiwish WM. Biosurfactant produced by the hydrocarbon-degrading bacteria: Characterization, activity and applications in removing TPH from contaminated soil. Environ Technol Innov. 2019; 14: 100347. DOI: https://faculty.uobasrah.edu.iq/uploads/publications/1617818987.pdf

Dayamrita KK, Divya KK, Sreelakshmi R, Arjun EJ, and John F. Isolation and characterization of hydrocarbon degrading bacteria from oil contaminated soil–Potential for biosurfactant assisted bioremediation. AIP Conference Proc. 2020; 2263(1): 020009. DOI: https://doi.org/10.1063/5.0017395

Adebajo SO, Akintokun AK, and Bolaji SE. Biosurfactants producing bacteria from oil-polluted soil in Abeokuta, Ogun state. Ife J Sci. 2018; 20(2): 287-297. DOI: https://doi.org/10.4314/ijs.v20i2.9

Habib S, Ahmad SA, Wan Johari WL, Abd Shukor MY, Alias SA, Smykla J, et al. Production of lipopeptide biosurfactant by a hydrocarbon-degrading Antarctic Rhodococcus. Int J mol sci. 2020; 21(17): 6138. DOI: https://doi.org/10.3390/ijms21176138

Anuraj N, Singh P, and Kumar Singh S. Screening, isolation and characterization of biosurfactant producing Bacillus subtilis strain ANSKLAB03. Bioinformation. 2018; 14(6): 304-314. DOI: https://doi.org/10.6026/97320630014304

Ansari N, Rokhbakhsh-Zamin F, Hassanshahian M, and Askari M. Study the effect of reducing the toxicity of oil pollution by crude oil-degrading bacteria symbiotic with the gastropod isolated from the persian gulf on the quality of seafood. QAFJ. 2021; 1(1): 73-86. DOI: 20.1001.1.27833410.1400.1.1.7.5

Bayat Z, Hassanshahian M, and Hesni MA. Enrichment and isolation of crude oil degrading bacteria from some mussels collected from the Persian Gulf. Mar Pollut Bull. 2015; 101(1): 85-91. DOI: https://doi.org/10.1016/j.marpolbul.2015.11.021

Nayarisseri, Anuraj, Poonam Singh, and Sanjeev Kumar Singh. Screening, isolation and characterization of biosurfactant producing Bacillus subtilis strain ANSKLAB03. Bioinformation. 2018; 14(6): 304. DOI: https://doi.org/10.6026/97320630014304

Mayank G, Priyanka, and Chatterjee M. Isolation, characterization and antibacterial effect of biosurfactant from Candida parapsilosis. Biotechnology Rep. 2018; 18: e00251. DOI: https://doi.org/10.1016/j.btre.2018.e00251

Milena M, Capão A, and Procópio L. Biosurfactant produced by oil-degrading Pseudomonas putida AM-b1 strain with potential for microbial enhanced oil recovery. Bioremediation J. 2019; 23(4): 302-310. Available at: https://www.cabdirect.org/cabdirect/abstract/ 20203339759

Zargar AN, Mishra S, Kumar M, and Srivastava P. Isolation and chemical characterization of the biosurfactant produced by

Gordonia sp. IITR100. Plos One. 2022; 17(4): e0264202. DOI: https://doi.org/10.1371/journal.pone.0264202

Yakimov MM, Timmis KN, and Golyshin PN. Obligate oil-degrading marine bacteria. Curr Opin Biotechnol. 2007; 18(3): 257-266. DOI: https://doi.org/10.1016/j.copbio.2007.04.006

Duru IA, Duru CE, Enyoh CE, and Umar HI. Computer-aided degradation susceptibility study of crude oil compounds at bacillus subtilis protein target. Environ Eng Res. 2022; 28(3): 210565. DOI: https://doi.org/10.4491/eer.2021.565

Kumar R and Jyoti Das A. Extraction, detection, and characterization of rhamnolipid biosurfactants from microorganisms. In: Rhamnolipid Biosurfactant. Springer, Singapore, 2018; p. 15-28. DOI: https://doi.org/10.1007/978-981-13-1289-2_2

Golamari Siva R, Siddiqui N, Sahitya P, Ayyappa K, Teja T, and Sivaramakrishna Akhil K. Isolation, screening, characterization and application of biosurfactant by achromobacter xylos strain GSR21 producing bacteria from hydrocarbons contaminated soil. Int J Life Sci Pharma Res. 2022; 12(1): 154-169.

Satpute KS, Mone NS, Das P, Banat IM, and Banpurkar AG . Inhibition of pathogenic bacterial biofilms on PDMS based implants by L. acidophilus derived biosurfactant. BMC microbiology. 2019; 19: 39. Available at: https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-019-1412-z

Gharaei S, Ohadi M, Hassanshahian M, Porsheikhali S, and Forootanfar H. Isolation, optimization, and structural characterization of glycolipid biosurfactant produced by marine isolate shewanella algae B12 and evaluation of its antimicrobial and anti-biofilm activity. Appl Biochem Biotechnol. 2022; 194(4): 1755-1774. DOI: https://doi.org/10.1007/s12010-021-03782-8

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